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1420 Introduction (.ppt)

The Model 1420 Scanning Video Microscope is a research-grade, inverted fluorescence video microscope for applications in many fields. This general purpose microscope combines some of the most advanced features with portability and value pricing. With a synchronously pulsed illuminator, sensitive 30-fps camera, and powerful video analysis software, the Model 1420 workhorse for many academic, industrial, R&D and research activities. Extensive filter, camera, illumination and objectives options let you tailor the system for your research.

Motorized traverse and focus, and software-configurable image-based "soft triggers" make the Model 1420 perfect for µTAS prototypes and miniature process automation. The Model 1420 is sized and priced to be dedicated to a single user or experiment, even in a crowded BSL-2 lab.

The Model 1420 microscope excels precisely where it counts: getting high-quality experimental data onto a hard drive. With fulling motorized optics, remote capabilities and an event trigger-n-store capability, the Model 1420 brings a variety of specialized features to everyday applications.

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Laboratory Automation

The Model 1420 can be configured seamlessly into a variety of laboratory applications. Our input and output options allow you to automate sample analysis, which increases throughput. Its motionless sample stage simplifies physical interfacing and ensures unperturbed microsystems. Motorized focus and X/Y traverse can be fully automated using LabView and the available software developers kit. Standard composite and S-Video analog outputs and USB and RS232 digital ports simplify electronic interfacing. Using the 4 digital input modules, the Model 1420 can be synchronized with digital controllers, a Model 575 pulse generator, interloc switches, sensors or many other devices. Remote operation is also handy for forensics laboratories, clean-room projects, or elements requiring special hanlding or storage conditions.

Features

•  A precision motorized microscope at mechanical scope pricing

•  30 second acquisition buffer with event triggering and storage

•  No mechanical controls or knobs to stress, just save your setup

•  Sensitive 30 fps color and B&W video cameras for low-light imaging

•  Synchronous pulsed illuminator fights blur and photo-bleaching

•  Bottom-up viewing and illumination

•  Motionless stage for unperturbed microsystems

•  Extensive optics, filter and camera options

•  Simple coordination with external apparatus

Applications

•  Lab Automation

•  Education and Academic Projects

•  Cleanroom Studies, Forensics

•  Incubator Research (remote)

•  Nanotechnology

•  Seperation Sciences

•  Microanalysis

•  Genetic Sciences

•  Life Sciences, Pathology Studies

•  Optical Bench Microscope

Key Specifications

•  Microscope Type - Inverted, Tabletop

•  x-y Traverse Resolution - 10 um

•  BW Camera Module - 640x480 pixels, 600 lines res

•  Color Camera Module -640x480 pixels, 490 lines res

•  Outputs: Frame and Rate Data to Strobe/Straddle

•  Small Footprint - Easy to Transport

•  Modular Design - Expand optics as needed

•  Objective —10X semi-plan (Standard)

•  Optional Objective —4X and 50X

•  Illuminator Module —4-ch array, 24 high-bright LEDs

Application Ideas

• Embed the Model 1420 and automate your µTAS.
• Motorized focus and x-ytraverse can be fully automated using LabVIEW and simple software developers kit (C++)
• Standard composite and S-Video analog outputs and USB and RS232 digital ports simplify electronic interfacing
• Motionless sample stage simplifies physical interfacing
• Losslessly record data to disk
• Post-save and instantly replay what just happened so you never “just missed it”
• Probe velocity by real-time particle image velocimetry
• Probe regions with real-time image processing
• Trigger devices and recording by hardware, software, and real-time image probes
• Sensitive 30 fps color or black & white video cameras support low-light imaging
• Synchronous pulsed illuminator fights blur and photo-bleaching
• Bottom up viewing and illumination and a motionless sample stage allow unhindered and unperturbed microsystem access
• Simple coordination with external apparatus µTAS research and prototyping
• Gene and protein chip reading
• General miniature lab automation
• Closed-loop microsystem control
• Automated methods development
• Array assays
• Forensics
• Blood analysis
• Particle image velocimetry (PIV)

Software Features

Losslessly record data to disk (640 x 480 pixels) - Post-save and instant replay so you never miss an event - Auto-acquisition by hardware, software, or real-time image processing - Track and trigger by local intensity or color - Analyze and trigger by high-resolution PIV.

Upgradeable

As with many of Berkeley Nucleonics’ products, the field upgrades and future features will integrate seamlessly into your Model 1420. We can offer simple options like switching between color and black & white cameras, or trying different illuminator colors. For more advanced customization, see our snap-in optical modules which are customizable or preconfigured for one or more fluorophores. The stage cover is machinable and is easily swapped to support custom fixturing. One-click firmware and software upgrades provide the latest features and capabilities.

The Model 1420 is compact (just 21 cm wide x 27 cm long x 9 cm tall) but scans up to 75 mm x 50 mm. With its size and price point, the Model 1420 is ideal for dedicating to a single user or experiment.

Camera Modules

The snap-in camera modules for the Model 1420 include a CCD camera, optional fluorescence filter and threading for standard DIN microscope objective:
Black and White Module: High sensitivity analog black & white camera with 640 x 480 pixels, 600 lines of resolution, 30 frames per second.

Color Module: Bayer-pattern analog color camera with 640 x 480 pixels, 490 lines of resolution, 30 frames per second, S-Video output.

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Camera Modules

The snap-in camera modules for the Model 1420 include a CCD camera, optional fluorescence filter and threading for standard DIN microscope objective:
Black and White Module: High sensitivity analog black & white camera with 640 x 480 pixels, 600 lines of resolution, 30 frames per second.

Color Module: Bayer-pattern analog color camera with 640 x 480 pixels, 490 lines of resolution, 30 frames per second, S-Video output.

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Illumination Options

The Model 1420 illuminator offers four selectable LED channels which can be used independently or simultaneously. You can specify the color for each channel from the following LED wavelengths:
Blue: 464-476 nm
Green: 520-535 nm
Red: 615-636 nm
White
Custom wavelengths are also available.

Filter Options

The SVM uses 12.5 mm diameter long pass filters. Standard filters from 515 nm to 610 nm are available.

Stage Plate Options

The standard stainless steel stage plate has a 50 x 75 mm opening for easy sample viewing. An optional plate is available with two openings of 20 x 32 mm and 22 x 66 mm for smaller chips.

Suggested Options for Typical Applications

The Model 1420 illuminator and filter options can be configured for particular applications. For example, when working with fluorescein particles the Model 1420 illuminator can be equipped with one channel of white LEDs (to view the channels and chip during setup) and three blue LED channels (to excite the fluorescein particles). A 530 nm filter and black & white camera are also suggested.